Objective of this research was to investigate the presence of antimicrobial compound as a secondary metabolite by the endophytes isolated from Alpinea galanga willd., rhizome. Isolation of endophytic fungi was carried out using screening methods. Characterization of the organism was performed morphologically and biochemically. Extraction and identification of secondary metabolite was performed using standard analytical methods. Antifungal activity was done using agar diffusion method. Nine different endophytes were isolated from rhizomes. One of the endophyte was identified as Aspergillus terrious MTCC11987. The culture was deposited at MTCC, Chandigarh, India. The organism was found to be positive for amylase, catalase and cellulase enzyme production. Antifungal activity against Saccharomyces cerevisiae and Candida albicans with zone diameters of 12.5 and 14 mm respectively were observed for the broth. The broth showed U.V. absorption maxima at 245 nm. TLC of the broth showed Rf of 0.72 near to reference sample. FTIR and 1H NMR data reveals the presence of lovastatin in open ring form in the isolated sample. This study confirms the ability of Aspergillus terrious MTCC11987 isolated from Alpine galanga to produce lovastatin. The statin produced was effective on Saccharomyces cerevisiae and Candida albicans. Hence it can be predicted that the antimicrobial and hypolipidimic activity of Alpinea galanga rhizomes is synergistically affected by the endophyte. Further the mechanism that exactly determines the synergism of the endophyte has to be derived.
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